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Original Research
7.
Molecular detection of virulence genes associated
with pathogenicity of Gram positive isolates
obtained from respiratory tract of apparently
healthy as well as sick goats -
Tushar Kisan Aher, Ashish Roy, Pranay Kumar
Vet World. 2012; 5(11): 676-681
doi:
10.5455/vetworld.2012.
676-681
Abstract
Aim: To know the prevalence of gram positive
isolates and their virulence genes obtained from
respiratory tract of apparently healthy as well as
sick goats.
Material and Methods: Nasal swabs
and tissue samples were screened for the presence of
microbial pathogens by cultural isolation,
biochemical confirmation. Further, these isolates
were subjected to the virulence gene detection by
PCR.
Results: Out of 144 isolates, 57
isolates of gram positive pathogens were obtained
and confirmed as Staphylococcus spp. (43 isolates)
and Streptococcus spp. (14 isolates) on the basis of
Gram staining, morphology, cultural identification
and biochemical characters. Five isolates (11.6%)
were found to be positive for Coagulase gene; 11
isolates (25.6%) were found to be positive for clfA
gene; and 14 isolates (32.6%) were found to be
positive for Spa gene.
Conclusion: The
presence of these genes confirmed the pathogenic
potential of gram positive pathogens and their
association with clinical manifestations in
respiratory tract infections of goats.
Keywords:
biochemical characters, PCR, respiratory tract of
Goat, Staphylococcus, Streptococcus, virulence genes
